HLA Typing with Sequence-Specific Oligonucleotide Primed PCR (PCR-SSO) and Use of the Luminex™ Technology
作者： Klara Dalva , Meral Beksac
DOI: 10.1007/978-1-59745-223-6_5
关键词:
摘要: The hybridization products obtained by PCR using sequence-specific oligonucleotides (PCR-SSO) can be traced either colorimetric- (streptavidin- biotin), X-ray-(digoxigenin-CSPD), or fluorescence- (FITC, PE) based detection systems. To achieve a faster, reliable, automated typing, microbead and fluorescence technology have been combined introduced to this field (XMAP technology). For each locus, maximum of 100 microspheres, which are recognizable their specific color originating from two internal fluorescent dyes, used. Each microsphere is coupled with single probe that capable hybridizing the biotin labeled complementary amplicon. Once occurs, it quantified via signal fluorescently (Streptavidin-PE) amplicons captured beads. Currently, there commercially available systems differ in scale probes method amplification denaturation. One these will described detail chapter.
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