Analysis of Mesorhizobium loti glycogen operon: Effect of phosphoglucomutase (pgm) and glycogen synthase (glgA) null mutants on nodulation of Lotus tenuis
作者： Viviana C. Lepek , Alejandra L. D'Antuono , Pablo E. Tomatis , Juan E. Ugalde , Susana Giambiagi
DOI: 10.1094/MPMI.2002.15.4.368
关键词:
摘要: The phosphoglucomutase (pgm) gene codes for a key enzyme required the formation of UDP-glucose and ADP-glucose, sugar donors biosynthesis glucose containing polysaccharides. A Mesorhizobium loti pgm null mutant obtained in this study contains an altered form lipopolysaccharide (LPS), lacks exopolysaccharide (EPS), β cyclic glucan, glycogen is unable to nodulate Lotus tenuis. nonnodulating phenotype was not due absence glycogen, since synthase (glgA) effectively nodulates legume. In M. loti, part metabolism cluster formed by GlgP (glycogen phosphorylase), glgB branching), glgC (ADP-glucose pyrophos-phorylase), glgA, pgm, glgX debranching). genes are transcribed as single transcript from glgP at least under control strong promoter (promoter I) upstream glgP. An alternative II), mapping 154-bp DNA fragment spanning 85 bp...
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