Cholesteryl sulfate: measurement with beta-sitosteryl sulfate as an internal standard.

摘要: A new method is described for the measurement of cholesteryl sulfate (CS) in plasma. Taking advantage use beta-sitosteryl (SS) as an internal standard it simpler and more rapid than methods currently use. It does not require radioactive isotopes. potential contamination CS band by free cholesterol excluded developing thin-layer chromatography plate on which sterols are first separated with a second solvent system pushes to front without appreciably moving band. The sensitive reproducible may be advantageous screening large populations. values obtained plasma concentrations normal subjects compare well those measured elaborate techniques.