Experimental Neospora caninum infection in chickens (Gallus gallus domesticus) with oocysts and tachyzoites of two recent isolates reveals resistance to infection.

摘要: Abstract The importance of birds in the biological cycle Neospora caninum is not clear. We report unsuccessful infection chickens (Gallus gallus domesticus) using two isolates N. caninum. In experiment #1, 30 White Leghorn were orally inoculated with viable oocysts (NC-SP1 isolate, 200 per bird) via crop at 21 days age. Groups three euthanised intervals 7 days (a total 9 weeks) and one group was challenged same oocyst dose 37 days p.i. observed for 11 weeks. Blood samples collected weekly, sera tested IFAT. Chicken tissues PCR, quantitative PCR immunohistochemistry. Two dogs approximately 45 days age fed from 138 159 days p.i. results indicated that resistant to neosporosis as revealed by failure seroconvert, detect parasite DNA or antigen immunohistochemistry bird tissues, no excretion avian tissues. Similar obtained #2, which 34 1-week-old each s.c. 100,000 tachyzoites NcWTDMn1 isolate on days 7, 15, 22, 28, 36 60 p.i. At necropsy, all serum collected. All remained asymptomatic, detected Seven day demonstrated low (1:25 dilution) levels antibodies agglutination test. 12-week-old pooled 10 did excrete oocysts. This investigation indicates are experimental