Long non-coding RNA urothelial carcinoma associated 1 induces cell replication by inhibiting BRG1 in 5637 cells.

摘要: Long non-coding RNA urothelial carcinoma associated 1 (UCA1) was first identified in bladder cancer tissues. High expression of UCA1 has suggested it may serve as a potential diagnostic molecular marker for cancer. Subsequent research cell lines showed that can promote proliferation, but the underlying mechanism remains unknown. In present study, we BRG1 binding partner using an vitro pull-down assay, and RNA-binding protein immunoprecipitation assay confirmed UCA1-BRG cells vivo. is chromatin remodeling factor with reported tumor suppressor activities directly upregulates levels p21 cycle inhibitor by sequences promoter. Depletion RNAi resulted upregulated inhibition replication, while overexpressed reduced promoted growth. Notably, downregulation induction proliferation antagonized function BRG1. highly expressed tissue samples are often high expression. Furthermore, found impairs both to promoter activity Collectively, these results demonstrate promotes inhibiting