Selenium improves the developmental ability and reduces the apoptosis in porcine parthenotes.

摘要: Selenium is an essential trace element in conventional tissue culture media to guarantee adequate biosynthesis of selenoprotein cellular antioxidant system protect the cells from oxidative damage and apoptosis. This study investigated effect selenium, form sodium selenite (SS), on developmental ability quality vitro produced porcine parthenotes. For this, parthenogenetic presumptive diploid zygotes were by electroactivation cultured absence or presence SS at different concentrations (0, 2.5, 25, 250 ng/ml) a serum-free defined medium supplemented with polyvinyl alcohol (PVA) bovine serum albumin (BSA). Results showed that, development rate 2–4 cell stage parthenotes blastocyst their number was increased while TUNEL index decreased, dose-dependent manner, when NCSU23 + PVA. Interestingly, approached those NCSU23 + BSA (P < 0.05), thereby suggesting PVA + 25 ng/ml be partial replacement BSA. In PVA, supplementation concentration 25 did not improve cleavage matured oocytes but there significant improvement (45.4 ± 8.8% vs. 12.7 ± 4.8%), total nuclei (42.1 ± 3.5 31.3 ± 2.9) inner mass (ICM) (29.4 ± 1.5% 21.3 ± 1.2%) decrease (5.6 ± 0.5 12.9 ± 1.3) compared nonsupplemented controls. The also decreased BAX:BCL-xL transcript ratio, expression ERK1/2 glutathione peroxidase (GPX) reduced level Caspase 3 proteins (P < 0.05). These data thus suggest that improves preventing Mol. Reprod. Dev. 74: 1386–1394, 2007. © 2007 Wiley-Liss, Inc.