Partial replacement of serum by selenite, transferrin, albumin and lecithin in haemopoitec cell cultures
作者： L. J. GUILBERT , N. N. ISCOVE
DOI: 10.1038/263594A0
关键词:
摘要: CULTURE media for mammalian cells usually require supplementation with serum to supply as yet undefined needs. Because the requirements are likely be multiple, it is difficult distinguish biologically between effects of nonspecific ‘nutritional’ factors in and those specific regulatory factors. Partial replacement these systems by chemically defined substances supplying needs would therefore represent a significant advance towards definition such We have examined role several components cultures freshly explanted haemopoietic this goal mind.
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