Evaluation of Thin Agar Layer Method for Recovery of Acid-Injured Foodborne Pathogens†

摘要: The thin agar layer (TAL) method of Kang and Fung was used to enumerate acid-injured foodborne pathogens. This involves overlaying 14 ml nonselective medium (tryptic soy [TSA]) onto a prepoured solidified pathogen-specific, selective in petri dish. After surface plating, injured cells resuscitated grew on TSA during the first few hours incubation; then, agents from diffused top layer, interacted with recovered microorganisms, started produce typical reactions. Foodborne pathogens were exposed 2% acetic acid for 1, 2, or 4 min, recovery rate TAL compared media. No significant difference occurred between (P > 0.05) enumeration Escherichia coli O157:H7, Salmonella Typhimurium, Staphylococcus aureus, Yersinia enterocolitica, both significantly higher numbers than ea...