Ca2+ modulates an unspecific cation conductance in olfactory cilia of Xenopus laevis
作者： D. Schild , J. Bischofberger
DOI: 10.1007/BF00231774
关键词:
摘要: Olfactory neurones of Xenopus laevis were studied by the patch clamp technique under voltage-clamp conditions. Isolated receptor cells obtained dissociating olfactory mucosa in a Ca2+-free solution. Usually some resulting isolated lost all their cilia during dissociation procedure. Comparing currents with to those without cilia, marked difference was found. When known voltage-gated except Ca2+-current blocked, showed alone whereas clearly had an additional conductance gc. It could be activated two ways, either Ca2+ entry through Ca2+-channels or Na/Ca-exchanger working reversed mode at positive membrane potentials. This ciliar gc its reversal potential 0 mV. Replacing extracellular Cl- isethionate on one hand, and Na+ Cs+ N-methyl-D-glucamine other that permeable for cations but not Cl-. In conclusion, there appears Ca2+-dependent unselective cation neurones. The probable role as last step IP3/Ca mediated transduction path-way is suggested.
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