Antigenic heterogeneity of the reticular meshwork in the white pulp of mouse spleen.

摘要: Monoclonal antibodies against cellular components of reticular meshworks were produced by immunizing rats with heterogeneous stromal-cell population mouse spleen. Immunohistochemical screening selected two antibodies, WP-1 and RPSC-2. proved to immunostain the meshwork B area densely, leaving marginal zone unstained; it also reacted sparsely T-cell region. In contrast, RPSC-2 selectively immunostained T Immuno-electron microscopy clearly visualized, for both reaction products being deposited along cytomembrane fibroblastic reticulum cells, their abundant cytoplasmic processes that densely intertwined lymphocytes. Double immunostaining followed divided white pulp into domains. The in region be immunostainable Thus, cells T-and B-cell areas, while indistinguishable routine microscopy, are at least partially heterogeneous.