Growth Differentiation Factor 9 (GDF9) Stimulates Proliferation and Inhibits Steroidogenesis by Bovine Theca Cells: Influence of Follicle Size on Responses to GDF9
作者： Leon J. Spicer , Pauline Y. Aad , Dustin T. Allen , Sabine Mazerbourg , Anita H. Payne
DOI: 10.1095/BIOLREPROD.107.063446
关键词:
摘要: Ovarian follicular development is controlled by numerous paracrine and endocrine regulators, including oocyte-derived growth differentiation factor 9 (GDF9), a localized increase in bioavailable insulin-like 1 (IGF1). The effects of GDF9 on function theca cells collected from small (3-6 mm) large (8-22 ovarian follicles were investigated. In small-follicle cultured the presence both LH IGF1, increased cell numbers DNA synthesis, as measured (3)H-thymidine incorporation assay, dose-dependently decreased progesterone androstenedione production. Theca had little or no response to terms proliferation steroid production induced IGF1. Small-follicle studies indicated that abundance LHR CYP11A1 mRNA cells, but effect IGF1R, STAR, CYP17A1 percentage staining for proteins. activated similar mothers against decapentaplegics (SMAD) 2/3-induced CAGA promoter activity transfected cells. more ALK5 than large-follicle granulosa appeared have greater detectable mRNA. We conclude are responsive those may be produced cattle. Because steroidogenesis, oocyte- cell-derived simultaneously promote prevent premature interna during early follicle development.
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