Vision Recovery and Connectivity by Fetal Retinal Sheet Transplantation in an Immunodeficient Retinal Degenerate Rat Model.
作者： Magdalene J. Seiler , Robert E. Lin , Bryce T. McLelland , Anuradha Mathur , Bin Lin
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摘要: Author(s): Seiler, Magdalene J; Lin, Robert E; McLelland, Bryce T; Mathur, Anuradha; Bin; Sigman, Jaclyn; De Guzman, Alexander Kitzes, Leonard M; Aramant, B; Thomas, Biju B | Abstract: PurposeTo characterize a recently developed model, the retinal degenerate immunodeficient S334ter line-3 rat (SD-Foxn1 Tg(S334ter)3Lav) (RD nude rat), and to test whether transplanted fetal sheets can elicit lost responses light.MethodsNational Institutes of Health rats Tg) with normal retina were compared RD without transplant for morphology visual function. Retinal from transgenic expressing human placental alkaline phosphatase (hPAP) into subretinal space between postnatal day (P) 26 P38. Transplant was examined in vivo using optical coherence tomography (OCT). Visual function assessed by optokinetic (OKN) testing, electroretinogram (ERG), superior colliculus (SC) electrophysiology. Cryostat sections analyzed various retinal/synaptic markers expression donor hPAP.ResultsOptical scans showed placement laminar development sheet transplants space. Optokinetic testing demonstrated deficit acuity that improved after transplantation. No ERG detected or Superior absent age-matched control sham surgery rats; however, robust light-evoked observed specific location SC rats. Responsive regions corresponded area eye. The quality correlated organization placement.ConclusionsThe data suggest integrate host recover significant
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