Lysis of Escherichia coli by induction of cloned ϕX174 genes
作者： B. Henrich , W. Lubitz , R. Plapp
DOI: 10.1007/BF00334146
关键词:
摘要: The largest of the fragments produced by AluI digestion ϕX174 RFI DNA comprises genes E and J as well parts D F. This fragment (1007 bp) was cloned into lac z′ gene plasmid pUR222. In recombinant pUH12, transcription is controlled p-o region. Induction addition inducer, IPTG, resulted in lysis bacteria. Cloning corresponding AluI-fragment from ϕX174am3 DNA, carrying an amber mutation E, showed that expression this alone sufficient to trigger cell lysis. time interval between IPTG onset depended on concentration however, rate similar at all concentrations used.
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