Mapping quantitative trait loci that influence blood levels of alkaline phosphatase in MRL/MpJ and SJL/J mice.

摘要: To examine the hypothesis that serum alkaline phosphatase (ALP) levels have a heritable component, we analyzed blood from two inbred strains of mice, MRL/MpJ and SJL, which exhibit 90% difference in total ALP activity (268 ± 26 vs. 140 15 U/l, respectively, P < 0.001). A genome-wide scan was carried out using 137 polymorphic markers 518 F2 female mice. Serum progeny showed normal distribution with an estimated heritability 56%. Genome-wide for cosegregation genetic marker data revealed three major quantitative trait loci (QTL), one each on chromosomes 2 (LOD score 3.8), chromosome 6 12.0), 14 3.7). In addition, there suggestive QTL 3.3). aggregate, these QTLs explain 22.5% variance between strains. moderate but significant correlation body weight adjusted bone mineral density (r = 0.12, 0.0108) periosteal circumference at midshaft tibia 0.15, 0.0006) The locus harboring also contains BMD size QTL, identified earlier, mice; this is close to regulates IGF-I 8-9) C3HB6 These common indicate observed or may be regulated by same (or genes). Accordingly, osteoblast cells isolated femur MRL mice significantly higher number +ve cells/colony two- threefold (P 0.001) as compared SJL thus suggesting differences reflect expression osteoblasts suggest are genetically determined correlate cellular mechanisms differentiate accrual findings traits share role determinants formation overall accretion.