Localization of Chromosome 9p Homozygous Deletions in Glioma Cell Lines with Markers Constituting a Continuous Linkage Group

摘要: Southern blot analyses of the 9p-localized type I interferon (IFN) genes in DNAs obtained from malignant glioma cell lines and glial tumor tissue have indicated that homozygous deletions IFN-alpha IFN-beta often occur during development highly central nervous system neoplasm, glioblastoma. We applied a set markers span IFN region on 9p to analysis 30 human order define deletion associated with this cancer more precisely. Fourteen revealed either complete (12 cases) or partial (2 gene cluster; no instances were observed did not involve region. Genomic DNA identified by nearest flanking retained 5 cases deletions. Consequently, these results limit extent small 9p21-p22 includes most locus.