Genomic Exploration on Chinese Hamster Ovary Cells

摘要: Industrially important recombinant CHO cell clones carry many desirable phenotypic traits that differentiate them from the parental clone. The molecular basis for these at genomic level is not well understood. A better understanding of key genetic alterations related to will greatly facilitate development new lines and processes. To advance this goal, we are constructing a cDNA microarray. phage library was constructed using mRNA pooled different under cultivation conditions. Over four thousand sequences have been randomly isolated sequenced. microarray based on used in gene expression studies. differentially expressed genes identified by were compared those observed cross-species hybridization mouse microarrays. Sequence comparison with known indicates only moderate degree homology. results represent significant step toward large-scale profiling industrial mammalian culture