Transport of osmoprotective compounds in hybridoma cells exposed to hyperosmotic stress.

摘要: Addition of osmoprotective compounds has a positive effect on growth and monoclonal antibody production in hyperosmotic hybridoma cell cultures. In order to better understand the processes involved response, uptake glycine betaine, proline, sarcosine mouse line 6H11 during exposure stress was studied. Hyperosmotic (510 mOsmol/kg) introduced through addition NaCl (100 mM) medium, amino acid transport activity measured immediately after transfer cells medium. The capability four osmoprotectants tested negatively affected if methylaminosobutyric (MeAiB), specific substrate for system A, simultaneously included medium equimolar amounts with one compounds. This due accumulation MeAiB stressed cells, giving significant reduction concentration compound inside cells. Furthermore, excess meAiB gave approx. 905 initial rate while 40–50% sarcosine. Similarly, or also betaine uptake. These results suggest that share, at least part, common, inhibitable carrier into osmotically is probably equal A.