Low-molecular-weight fucoidan protects endothelial function and ameliorates basal hypertension in diabetic Goto-Kakizaki rats

摘要: Endothelial dysfunction, characterized by impairment of endothelial nitric oxide synthase (eNOS) and (NO) bioavailability, has been implicated in diabetic cardiovascular pathogenesis. In this study, low-molecular-weight fucoidan (LMWF), which multiple biological activities including anti-inflammatory anti-oxidative properties, was investigated for its protective effect against dysfunction Goto-Kakizaki type 2 rats. LMWF (50, 100, or 200 mg/kg/day) probucol (100 mg/kg/day) were given to rats 12 weeks. Basal blood pressure, acetylcholine- flow-mediated relaxation mesenteric paw arteries, endothelium-dependent dilation aorta, eNOS phosphorylation, NO production measured using laser Doppler flowmetry, force myograph, hematoxylin eosin staining, western blot analysis, an assay. We found that robustly ameliorated the basal hypertension as well arteries addition, reduction phosphorylation at Ser1177, expression, because diabetes partially reversed treatment. However, probucol, a lipid-modifying drug with antioxidant displayed only mild effects. Moreover, induced, dose-dependent manner, vasodilation Ser1177 normal also promoted synthesis primary cultured vasoendothelial cells. Thus, these data demonstrate first time protects function reduces pressure via, least part, preservation function. Fucoidan is therefore potential candidate protection endothelium complications.