Possible involvement of hippocampal immediate-early genes in contextual fear memory deficit induced by cranial irradiation.

摘要: Abstract Cranial irradiation can trigger adverse effects on brain functions, including cognitive ability. However, the cellular and molecular mechanisms underlying radiation-induced impairments remain still unknown. Immediate–early genes (IEGs) are implicated in neuronal plasticity related functions (i.e., memory formation) hippocampus. The present study quantitatively assessed changes mRNA protein levels of learning-induced IEGs, Arc , c-fos zif268 mouse hippocampus after cranial using quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) immunohistochemistry, respectively. Mice (male, 8-week-old C57BL/6) received whole-brain with 0 or 10 Gy gamma-ray and, 2 weeks later, contextual fear conditioning (CFC) was used to induce IEGs. In CFC task, mice evaluated exhibited significant deficits compared sham (0 Gy)-irradiated controls. encoding IEGs were significantly upregulated 10 30 min training. irradiated hippocampi lower than those sham-irradiated IEG increased all hippocampal regions, dentate gyrus, cornu ammonis (CA)1, CA3, CFC-induced upregulation 10 Gy-irradiated that controls, although there no differences between hippocampi. Thus, impairs induction (particularly ) via behavioral memory, this disturbance may be associated evident irradiation, possibly through dysregulation during formation.