Early assessment of leukocyte alterations at diagnosis of septic shock.

摘要: A dramatic decrease in circulating lymphocyte number is regularly described after septic shock. However, it unknown how early this alteration develops diagnosis of shock and if remains stable over time. Twenty-one patients with no comorbidities were included within 2 h the beginning vasopressive treatment. Flow cytometry phenotyping leukocyte subpopulations quantitative real-time polymerase chain reaction T-bet, GATA-3, FOXP3, RORγ mRNA performed from every 6 during subsequent 48 h. From their admission intensive care unit, present major alterations count (leukocytosis, neutrophilia, lymphopenia). The numbers (T, B, natural killer cells) diminished. Gene expression analysis transcription factors specific for TH1, TH2, CD4CD25 regulatory, TH17 lymphocytes showed a severe comparison healthy individuals' values. These remain first inclusion protocol despite aggressive resuscitation antibiotherapy administered patients. At time already lymphopenia involving subsets including CD4 T-cell subpopulations. No significant variation could be detected This should taken into account forthcoming clinical trials testing immunomodulating therapies