Caspase inhibition and limitation of myocardial infarct size: protection against lethal reperfusion injury
作者： D M Yellon , M M Mocanu , G F Baxter
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摘要: Ischaemia-reperfusion injury causes cell death by both necrosis and apoptosis. Caspase activation is a major event in We therefore examined the effect of caspase inhibitors during reperfusion upon myocardial infarction. Rat isolated hearts were subjected to 35 min coronary occlusion 120 reperfusion. Treatment groups perfused with early assessed non-selective inhibitor (Z-VAD. fmk, 0.1 microM), caspase-8 (Z-IETD.fmk, 0.07 caspase-9 (Z-LEHD.fmk, microM) caspase-3 (Ac-DEVD.cmk, microM). All limited infarct size (infarct-risk ratio per cent: control 38.5+/-2.6; Z-VAD. fmk 24.6+/-3.4; Z-LEHD.fmk 19.3+/-2.4; Z-IETD.fmk 23.0+/-5.4; Ac-DEVD.cmk 27.8+/-3.3; P<0.05 when compared value, 1-way ANOVA). conclude that inhibition protects myocardium against lethal injury.
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