Heartwood extract of Rhus verniciflua Stokes and its active constituent fisetin attenuate vasoconstriction through calcium-dependent mechanism in rat aorta.

摘要: Rhus verniciflua Stokes (RVS) exert cardiovascular protective activity by promoting blood circulation, but its active ingredients and underlying mechanism have yet to be identified. This study investigated the vascular effects of RVS, focusing on vasoconstriction smooth muscle Ca(2+) signaling. RVS heartwood extract attenuated contraction aortic rings induced vasoconstrictors serotonin phenylephrine, inhibited signaling evoked in cells. Subsequent activity-guided fractionation identified fisetin as an constituent exerting a inhibitory effect. Fisetin could inhibit major mobilization pathways including extracellular influx mediated L-type voltage-gated channel, release from intracellular store store-operated entry. In accordance with effect, phenylephrine. These results suggest that anticontractile which is presumably inhibition signaling, may contribute improvement circulation RVS.