The epidermal growth factor precursor isolated from murine kidney membranes. Chemical characterization and biological properties.
作者： J A Breyer , S Cohen
DOI: 10.1016/S0021-9258(17)46260-0
关键词:
摘要: To understand the biology and biochemistry of epidermal growth factor (EGF) precursor in normal tissues we partially purified EGF from mouse kidney. The was by affinity chromatography, using wheat germ lectin antibodies to murine EGF. is a glycosylated integral membrane protein apparent molecular mass 140-150 kDa. solubilized biologically active as evidenced its ability compete with 125I-labeled for binding receptor intact fibroblasts stimulate cells dependent on growth. kidney can be proteolytically processed EGF-associated arginine esterase into smaller fragment (97 kDa) that retains both immunologic sensitivity antiserum biological activity. Extensive digestion pepsin liberates immunologically approximately size mature
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