Enhanced anti-tumor immunity by superantigen-pulsed dendritic cells

摘要: Staphylococcal enterotoxins A (SEA) and B (SEB) are classical models of superantigens (SAg), which induce potent T-cell-stimulating activity by forming complexes with MHC class II molecules on antigen-presenting cells. This large-scale activation T-cells is accompanied increased production cytokines such as interferon-γ (IFN-γ). Additionally, we previously reported, IFN-γ-producing CD8+ T cells act “helper cells,” supporting the ability dendritic to produce interleukin-12 (IL-12)p70. Here, show that DC pulsed SAg promote enhancement anti-tumor immunity. Murine bone marrow-derived (DC) were OVA257–264 (SIINFEKL), an H-2Kb target epitope EG7 [ovalbumin (OVA)-expressing EL4] cell lines, in presence SEA SEB subcutaneously injected into naive C57BL/6 mice. plus OVA257–264-pulsed vaccine strongly enhanced peptide-specific exhibiting OVA257–264-specific cytotoxic IFN-γ production, leading induction protective immunity against tumors. Furthermore, cyclophosphamide (CY) added tumor-antigens (OVA257–264, tumor lysate, or TRP-2) immunization markedly tumor-specific T-cell expansion had a significant therapeutic effect various tumors (EG7, 2LL, B16). Superantigens potential candidates for enhancing vaccines.