Bovine cytokine expression during different phases of bovine leukemia virus infection
作者： Robert G. Keefe , Yeon Choi , David A. Ferrick , Jeffrey L. Stott
DOI: 10.1016/S0165-2427(96)05727-3
关键词:
摘要: Abstract The potential role of aberrant cytokine production in the pathogenesis bovine leukemia virus (BLV) was studied by analyzing mRNA expression pokeweed-stimulated PBMLs cows different phases disease progression. To analyze mRNA, a semi-quantitative RT-PCR assay developed. developed for detection IL-2, -4, -6, -10, -12, IFN-γ and actin using cDNA derived from phorbol-stimulated peripheral blood mononuclear leukocytes. Using PCR specific BLV tax, agar gel immunodiffusion white cell counts, BLV-negative, BLV-positive aleukemic (AL), persistently lymphocytotic (PL) cattle were identified. Peripheral lymphocytes cultured vitro 24 h pokeweed mitogen analyzed assay. Consistently elevated levels IL-2 IL-12 AL PL mitogen-stimulated cells detected, while but not cattle.
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