Integrated bovine leukosis proviral DNA in T helper and T cytotoxic/suppressor lymphocytes

摘要: Bovine leukosis virus (BLV) is associated with the disease complex enzootic bovine leukosis. The infection may remain clinically silent in form of an aleukaemic state or emerge as a persistent lymphocytosis and more rarely lymphosacroma. BLV has been considered classically to be B lymphotropic virus, based upon absolute increase lymphocytes lymphocytosis, lymphocyte phenotype majority cells making up lymphosarcomas identification viral antigen expressed following vitro culture peripheral blood mononuclear leukocytes. This association well established but mechanism(s) expression not defined. To examine further cellular tropism(s) BLV, T subpopulations from 10 lymphocytotic cattle were vitro. Lymphocyte cultures characterized by their subpopulation DNA was extracted for integrated provirus Southern blot hybridization. Provirus identified derived seven cattle, both helper cytotoxic/suppressor affected.