Spermatogenic onset. II: FSH modulates mitotic activity of germ and Sertoli cells in immature rats
作者： ISABEL ALMIRON , H. CHEMES
DOI: 10.1111/J.1365-2605.1988.TB00998.X
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摘要: Summary By using high doses of testosterone propionate (TP) endogenous FSH was lowered to non-detectable levels in immature rats different ages. Combined administration TP and human menopausal gonadotrophin (hMG) or purified (hFSH) restored circulating normal supranormal levels. This experimental model used investigate the influences hormones on proliferation Sertoli germ cells. Mitoses seminiferous tubules were counted after being blocked by colchicine. The mitotic index so determined showed a reduction with withdrawal significant increase when hormone restored. Testicular DNA content testicular homogenates similar changes. Autoradiographs prepared 3H-thymidine incorporation into cells quantified. It found that hFSH induced labelling indices gonocytes, type A-spermatogonia All these changes effective younger than 10 days but no modifications any parameters under study observed 20 days. is concluded exerts stimulatory effect spermatogonia, during first life.
sci-hub.se 参考文章(25)
E. STEINBERGER, A. STEINBERGER, M. FICHER, Study of spermatogenesis and steroid metabolism in cultures of mammalian testes. Recent Progress in Hormone Research. ,vol. 26, pp. 547- 588 ,(1970) , 10.1016/B978-0-12-571126-5.50018-2
Duckett Ge, Steinberger E, Hormonal control of spermatogenesis. Reproduction. ,(1967)
ISABEL ALMIRÓN, HORACIO DOMENÉ, HÉCTOR E. CHEMES, The hormonal regulation of premeiotic steps of spermatogenesis in the newborn rat. Journal of Andrology. ,vol. 5, pp. 235- 242 ,(1984) , 10.1002/J.1939-4640.1984.TB00784.X
FC GREENWOOD, WM HUNTER, JS GLOVER, THE PREPARATION OF 131I-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY Biochemical Journal. ,vol. 89, pp. 114- 123 ,(1963) , 10.1042/BJ0890114
H. E. Chemes, M. Dym, H.G.M. Raj, The Role of Gonadotropins and Testosterone on Initiation of Spermatogenesis in the Immature Rat Biology of Reproduction. ,vol. 21, pp. 241- 249 ,(1979) , 10.1095/BIOLREPROD21.1.241
Michael D. Griswold, Alberto Solari, Pierre S. Tung, Irving B. Fritz, Stimulation by follicle-stimulating hormone of DNA synthesis and of mitosis in cultured Sertoli cells prepared from testes of immature rats Molecular and Cellular Endocrinology. ,vol. 7, pp. 151- 165 ,(1977) , 10.1016/0303-7207(77)90064-8
A. Fleck, H.N. Munro, The precision of ultraviolet absorption measurements in the Schmidt-Thannhauser procedure for nucleic acid estimation Biochimica et Biophysica Acta. ,vol. 55, pp. 571- 583 ,(1962) , 10.1016/0006-3002(62)90836-3
Yves Clermont, Annick Mauger, Existence of a spermatogonial chalone in the rat testis. Cell Proliferation. ,vol. 7, pp. 165- 172 ,(1974) , 10.1111/J.1365-2184.1974.TB00408.X
Yves Clermont, Bernard Perey, Quantitative study of the cell population of the seminiferous tubules in immature rats American Journal of Anatomy. ,vol. 100, pp. 241- 267 ,(1957) , 10.1002/AJA.1001000205
JOANNE M. ORTH, The role of follicle-stimulating hormone in controlling Sertoli cell proliferation in testes of fetal rats. Endocrinology. ,vol. 115, pp. 1248- 1255 ,(1984) , 10.1210/ENDO-115-4-1248