Detection of tumor-specific genetic alterations in bone marrow from early-stage breast cancer patients.

摘要: Detection of genetic markers associated with early breast cancer may prove clinically relevant for identifying patients at increased risk relapse. Loss heterozygosity (LOH), a specific aberration, commonly occurs during initiation and metastasis. Early detection tumor metastasis to bone marrow (BM) using conventional histochemical techniques has been limited because suboptimal efficiency sensitivity. Because is such common site recurrence, we sought determine whether microsatellite could be detected in BM aspirates from early-stage cancer. Cell-free plasma 48 was assessed LOH panel eight polymorphic markers. 11 (23%) patients’ aspirates. Advancing American Joint Committee on Cancer (AJCC) stage an incidence LOH. Concordance present between identified matched-pair primary tumors. No samples contained detectable cells routine histology. In 24 patients, paired peripheral blood serum were available analysis. these cases, more frequent than serum. This study demonstrates the novel finding tumor-related aspirate provides unique approach assessing subclinical systemic disease progression monitoring patients.