Mitogenic Factors from Group G Streptococci Associated with Scarlet Fever and Streptococcal Toxic Shock Syndrome

摘要: Since 1987, over 30 group G streptococcus (GGS) clinical isolates have been referred to our laboratory and 3 investigated for production of streptococcal pyrogenic exotoxins(SPEs) A, B, C. One isolate was associated with scarlet fever two others toxic shock syndrome(STSS). All three were grown in culture shown not produce SPEs C by double immunodiffusion Western blotting ethanol precipitated, concentrated fluid. Isolated DNA from these organisms, separated agarose gel electrophoresis, blotted on nylon membranes developed a digoxigenin detection system, failed hybridize probes specific the genes speA, speB, speC. We next antigenically distinct mitogens subjecting ammonium sulfate or precipitated fluids isoelectric focusing(IEF) assaying fractions IEF mitogenicity use rabbit splenocyte proliferation assay. showed peaks mitogenic activity. The largest activity one assayed pyrogenicity ability enhance lethal lipopolysaccharide (LPS) model. After injection sample, rabbits observed 4 hours then given sub-lethal LPS. exhibited but none died. In an earlier experiment, all 2/3 These results indicate that GGS factors which are could be novel superantigens.