Separation of purine and pyrimidine bases by ion chromatography with direct conductivity detection
作者： Lu Liu , Jin Ouyang , Willy R.G. Baeyens
DOI: 10.1016/J.CHROMA.2008.04.011
关键词:
摘要: Abstract A simple, rapid and accurate method for the simultaneous determination of four purine pyrimidine bases (cytosine, 5-methylcytosine, adenine N6-methyladenine) has been developed. The quantitative these was accomplished by ion chromatography (IC) with direct conductivity detection (CD) based on their ionization in acidic medium without chemical suppression. recovery cytosine, calf thymus DNA more than 98% (n = 3) relative standard deviation (RSD, n = 5) less 2.4%. In a single chromatographic run, could be separated determined 10 min. limits were found to 0.05 μg/mL 0.08 μg/mL 0.07 μg/mL adenine, N6-methyladenine. Linear ranges 0.2–95.1 μg/mL cytosine (r2 = 0.9996), 0.3–196.6 μg/mL 5-methylcytosine (r2 = 0.9994), 0.3–105.5 μg/mL (r2 = 0.9998), 0.3–159.1 μg/mL N6-methyladenine (r2 = 0.9999). With proposed method, successfully detected DNA. We also using RP-HPLC. Compared RP-HPLC, IC offers advantages such as high selectivity simple mobile phase.
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sci-hub.se 参考文章(16)
Gajanan V. Sherbet, DNA methylation and genetic instability Genetic Recombination in Cancer. pp. 133- 153 ,(2003) , 10.1016/B978-012639881-6/50008-7
Suhui Shen, Jin Ouyang, Willy R.G. Baeyens, Joris R. Delanghe, Yiping Yang, Determination of β2-agonists by ion chromatography with direct conductivity detection Journal of Pharmaceutical and Biomedical Analysis. ,vol. 38, pp. 166- 172 ,(2005) , 10.1016/J.JPBA.2004.11.060
Y.X. Gong, S.P. Li, P. Li, J.J. Liu, Y.T. Wang, Simultaneous determination of six main nucleosides and bases in natural and cultured Cordyceps by capillary electrophoresis. Journal of Chromatography A. ,vol. 1055, pp. 215- 221 ,(2004) , 10.1016/J.CHROMA.2004.09.015
Li Yu, Jing Zhao, Shao Ping Li, Hui Fan, Min Hong, Yi Tao Wang, Quan Zhu, None, Quality evaluation of Cordyceps through simultaneous determination of eleven nucleosides and bases by RP-HPLC Journal of Separation Science. ,vol. 29, pp. 953- 958 ,(2006) , 10.1002/JSSC.200600007
Gang Chen, Xiaohui Han, Luyan Zhang, Jiannong Ye, Determination of purine and pyrimidine bases in DNA by micellar electrokinetic capillary chromatography with electrochemical detection Journal of Chromatography A. ,vol. 954, pp. 267- 276 ,(2002) , 10.1016/S0021-9673(02)00161-9
Richard R. Meehan, DNA methylation in animal development. Seminars in Cell & Developmental Biology. ,vol. 14, pp. 53- 65 ,(2003) , 10.1016/S1084-9521(02)00137-4
James S. Fritz, Factors affecting selectivity in ion chromatography. Journal of Chromatography A. ,vol. 1085, pp. 8- 17 ,(2005) , 10.1016/J.CHROMA.2004.12.087
Bruce Richardson, DNA methylation and autoimmune disease Clinical Immunology. ,vol. 109, pp. 72- 79 ,(2003) , 10.1016/S1521-6616(03)00206-7
Ngoc-Phuc Hua, Takeshi Naganuma, Application of CE for determination of DNA base composition. Electrophoresis. ,vol. 28, pp. 366- 372 ,(2007) , 10.1002/ELPS.200600318
G Xu, C Di Stefano, H.M Liebich, Y Zhang, P Lu, Reversed-phase high-performance liquid chromatographic investigation of urinary normal and modified nucleosides of cancer patients. Journal of Chromatography B: Biomedical Sciences and Applications. ,vol. 732, pp. 307- 313 ,(1999) , 10.1016/S0378-4347(99)00296-0