Regulation of neurite outgrowth mediated by localized phosphorylation of protein translational factor eEF2 in growth cones

摘要: Nerve growth cones contain mRNA and its translational machinery thereby synthesize protein locally. The regulatory mechanisms in the cone, however, remain largely unknown. We previously found that calcium entry-induced increase of phosphorylation eukaryotic elongation factor-2 (eEF2), a key component translation, within showed arrest neurites. Because dephosphorylated eEF2 phosphorylated are known to promote inhibit respectively, data led hypothesis eEF2-mediating translation may regulate neurite outgrowth. Here, we validated by using chromophore-assisted light inactivation (CALI) technique examine roles localized kinase (EF2K), specific calmodulin-dependent enzyme for phosphorylation, advancing cultured chick dorsal root ganglion (DRG) neurons. was weakly distributed cones, whereas increased extracellular adenosine triphosphate (ATP)-evoked transient through P2 purinoceptors resulted inhibition phosphatase 2A dephosphorylate also CALI retardation outgrowth, EF2K enhanced outgrowth temporally. Moreover, abolished ATP-induced These findings suggest an state cone regulates © 2012 Wiley Periodicals, Inc. Develop Neurobiol, 2013