Lactobacilli in the gastrointestinal tract of dog and wolf

摘要: Since the first wolf was taken as a companion animal lot have happened with biology of these animals. Is it possible that changes in gut microbiota domestic dog are involved increasing numbers animals gastrointestinal disorders? Probiotics been proven to promote immune system dogs by concentrations neutrophils and monocytes, decreasing fragility erythrocytes serum IgG concentrations. also shown improve fecal consistency, dry matter defecation frequency dogs. The aim this study find out if bacterial counts differed from wolves, focus on lactobacilli. presence Lactobacillus reuteri characterization such strains wolves analyzed probiotic candidate. Fecal specimens five intestine content four were collected lactobacilli isolated cultivation selective substrate Rogosa. Bacterial enumerations noted colonies morphological differences before done using reuterin assay, rep-PCR gel electrophoresis. A representative for each type identified 16S rRNA gene sequencing. Bacteria L. characterized further methods testing bile tolerance, detection genes ureC pduC, mucus binding capacity antibiotic susceptibility. handful isolates determining sequence cell surface protein specific reuteri. samples ranged 106 108 cfu g-1 feces 103 105 content. Nevertheless, not all bacteria grown Rogosa agar lactobacilli, species Streptococcus isolated. Even though total higher dog, number different wolf. In two isolated; animalis while another 32 subjects could be divided into 10-12 according methods. Among 13 methods, positive urease positive. Some bound well intestinal others did bind at all. tolerance between but most them, except one strain, survived concentration 5% bile. results susceptibility test showed close relation strains, resistance against streptomycin tetracycline. both whereas other only found or This established analyzing referred “protein x”. new method isolating tested, called immunomagnetic isolation. negative, since beads some Dynabeads sample any sample.