Comparative Genomics of Thalassobius Including the Description of Thalassobius activus sp. nov., and Thalassobius autumnalis sp. nov.

摘要: A taxogenomic study was conducted to describe two new Thalassobius species and analyze the internal consistency of genus along with Shimia Thalassococcus. Strains CECT 5113T, 5114, 5118T 5120 were isolated from coastal Mediterranean seawater, Spain. Cells Gram-negative, non- motile coccobacilli, aerobic chemoorganotrophs, an optimum temperature 26oC salinity 3.5-5%. Major cellular fatty acids strains 5113T 5114 C18:1 7c/6c C10:0 3OH, G+C content 54.4-54.5 mol% able utilize propionate, L-threonine, L- arginine L-aspartate as carbon sources. They exhibited 98.3% 16S rRNA gene sequence similarity, 75.0-75.1 ANIb 19.5-20.9 in silico DDH type strain their closest species, maritimus. Based on these data, are recognized a for which name activus is proposed, (=LMG 29900T) strain. found constitute another major 7c 11-methyl 59.8 mol%; they not or L-aspartate. Their mediterraneus, values 99.6% 79.1% 23.2% compared strain, 5383T. The autumnalis proposed this second 29904T) To better determine phylogenetic relationship we submitted twelve genomes representing Thalassobius, Thalassoccocus, phylogenomic analysis based 54 single protein-encoding genes (BCG54). resulting tree did agree current genera classification, divided three clades, sensu stricto (T. T. sp. nov. gelatinovorus), aestuarii plus spp (S. marina, S. haliotis SK013) finally, Thalasobius maritimus Thalassococcus halodurans remained apart genera. Phenotypic inferences explored presented.