Use of latex agglutination test to determine rabies antibodies in production of rabies antisera in horses.
作者： Wachiraporn Saengseesom , Songsri Kasempimolporn , Visith Sitprija , Surasak Akesowan , Suraseha Ouisuwan
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摘要: A therapeutic anti-rabies immunoglobulin for human use has been produced mainly in horses. The presently available seroneutralization test, the rapid fluorescent focus inhibition test (RFFIT), is laborious and rather difficult to carry out horse farms. This study was undertaken develop a simple latex agglutination (LAT) determining rabies antibodies sera. LAT validated by testing total of 468 serum samples characterized RFFIT. Of these, 253 260 with antibody titers less than 100 IU/ml had score 1+, whereas 174 208 equal or greater scores 2-4+. Results correlated those RFFIT (r = 0.87, p < 0.0001). advantages being rapid, perform, easy interpret, applicable as an on-site tool estimation
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