作者: Michael Chang , Mohammed Bellaoui , Chaoying Zhang , Ridhdhi Desai , Pavel Morozov
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摘要: SGS1 encodes a DNA helicase whose homologues in human cells include the BLM, WRN, and RECQ4 genes, mutations which lead to cancer-predisposition syndromes. Clustering of synthetic genetic interactions identified by large-scale network analysis revealed that interaction profile gene RMI1 (RecQ-mediated genome instability, also known as NCE4 YPL024W) was highly similar TOP3, suggesting functional relationship between Rmi1 Sgs1/Top3 complex. We show physically interacts with Sgs1 Top3 is third member this Cells lacking activate Rad53 checkpoint kinase, undergo mitotic delay, display increased relocalization recombination repair protein Rad52, indicating presence spontaneous damage. Consistent role for maintaining integrity, rmi1Δ exhibit frequency gross chromosomal rearrangements. In addition, strains fail fully upon exposure DNA-damaging agents, an important part Rad53-dependent damage response.