Using Liposomes as Carriers for Polyphenolic Compounds: The Case of Trans-Resveratrol

摘要: Resveratrol (3,5,4′-trihydroxy-trans-stilbene) is a polyphenol found in various plants, especially the skin of red grapes. The effect resveratrol on human health topic numerous studies. In fact this molecule has shown anti-cancer, anti-inflammatory, blood-sugar-lowering ability and beneficial cardiovascular effects. However, for many compounds natural origin bioavailability limited by low solubility biological fluids, as well rapid metabolization vivo. Therefore, appropriate carriers are required to obtain efficient therapeutics along with administration doses. Liposomes excellent candidates drug delivery purposes, due their biocompatibility, wide choice physico-chemical properties easy preparation. In paper liposome formulations made saturated phosphatidyl-choline (DPPC) cholesterol (or its positively charged derivative DC-CHOL) were chosen optimize loading rigid hydrophobic such resveratrol. Plain loaded liposomes characterized size, surface charge structural details complementary techniques, i.e. Dynamic Light Scattering (DLS), Zeta potential Small Angle X-ray (SAXS). Nuclear Electron Spin magnetic resonances (NMR ESR, respectively) also used gain information at molecular scale. The obtained results allowed give an account which interacted bilayer, being more deeply inserted cationic than zwitterionic liposomes. Relevant mean size presence oligolamellar structures influenced RESV guest molecules. The toxicity all these systems was tested stabilized cell lines (mouse fibroblast NIH-3T3 astrocytes U373-MG), showing that viability not affected liposomial resveratrol.