Gluconeogenesis in the Isolated Perfused Rat Kidney

摘要: Abstract The perfused rat kidney was used for a study of gluconeogenesis. completely isolated and with Krebs-Henseleit bicarbonate buffer containing 5% albumin. Perfusion flow rate pressure remained stable 2 to 3 hours, although the glomerular filtration diminished time. fractional reabsorption both water sodium 0.92 during 1st hour perfusion. glucose formation from pyruvate linear in excess 1 µmole per min g tissue. Glucose production l-lactate less than that pyruvate, uptake lactate also less. Fructose converted more readily dihydroxyacetone, at approximately same as pyruvate. l-glutamate proceeded about l-lactate. l-Alanine produced no glucose, alanine could be detected. A mixture amino acids, l-lactate, glycerol normal plasma concentrations supported gluconeogenesis an initial 0.6 kidney. sensitive small changes pH perfusate. With either lactate, glutamine, or substrate predominantly greater 7.23 7.64. Conversely, fructose inhibited by increased Addition adenosine 3',5'-monophosphate perfusate stimulated but not glycerol. Neither glucagon, epinephrine, parathyroid extract, nor vasopressin altered