Optimization of Saccharomyces cerevisiae culture in alginate-chitosan-alginate microcapsule

摘要: Microencapsulation is one of the promising methods for microorganism immobilization. The parameters microencapsulation have impact on growth and performance microencapsulated microorganism. In this paper, effects microcapsules core state (solid or liquid), initial cell density (1.5 x 10(7), 3 10(6) 10(5) cells/ml microcapsules), microcapsule diameter (200, 500, 600 700 mu m) membrane formation times (0, 5, 15 30 min) growth, including proliferating capacity, metabolic activity product secretion Saccharomyces cerevisiae, cultured in alginate-chitosan-alginate (ACA) microcapsule, were investigated. results showed that there was no significant difference cells, whether solid liquefied. Increase inoculate shortened lag phase time while obtained 25 which highest. size had proliferation, metabolism secretion, but leakage bacteria least when m diameter. increase reduced cells. It demonstrated optimized S. cerevisiae culture diameter, min. This study provides useful information intestinal delivery therapeutic agents from genetically modified food-grade microorganisms, using microencapsulation. (c) 2005 Elsevier B.V. All rights reserved.