Full length genomes of genotype IIIA Hepatitis A Virus strains (1995-2008) from India and estimates of the evolutionary rates and ages.

摘要: Abstract With the changing epidemiology, outbreaks of Hepatitis A Virus (HAV) have been reported from different parts India. To characterize HAV strains circulating in India (1995–2008), 6 full genome sequences predominant genotype, IIIA, were determined. Further, applying Bayesian Markov Chain Monte Carlo (MCMC) framework to genomes Indian as well other global (human simian), we derived mean nucleotide substitution rate and evolutionary timescales with emphasis on age genotype III IIIA strains. The genomic length all isolates was 7464 nt excluding poly tract. Phylogenetic analysis confirmed that close Nor-21 ( AJ299464 ) HMH AY644337 subgenotype IIIA. ORF when compared within at amino acid level showed a highly conserved pattern. Under best fit expansion population relaxed molecular clock model, estimated simian strains) 1.73 × 10 −4 substitutions/site/year based which earliest transmission humans is occurred about 3564 years ago. human under same model be 1.99 × 10 substitutions/site/year. this 592 while 202 years. time most common recent ancestor (tMRCA) calculated 116