Production and secretion of human interleukin-18 in transgenic tobacco cell suspension culture
作者： Niti Sharma , Tae-Geum Kim , Moon-Sik Yang
DOI: 10.1007/BF02931900
关键词:
摘要: Interleukin-18 (IL-18), otherwise known as interferon-gamma-inducing factor (IGIF), is one of several well characterized and important cytokines that contribute to host defenses. The complementary DNA (cDNA) mature human interleukin-18 gene (hIL-18) was fused with the signal peptide rice amylase 1A (Ramy1A) introduced into plant expression vector under control a duplicated CaMV 355 promoter. recombinant plasmid transformed tobacco (Nicotiana tabacum L. cv Havana) using theAgrobacterium-mediated transformation method. integration hIL-18 genome transgenic plants confirmed by polymerase chain reaction (PCR) amplification its observed in suspension cells were derived from callus Northern blot analysis. protein detected extracts medium culture immunoblot Based upon enzyme-linked immunosorbant assay (ELISA) results, level approximated 166 μg/L medium. Bioassay results induction interferon-γ KG-1 cell line indicated secreted bioactive.
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Bin Zhang, Ying-Hua Yang, Yong-Ming Lin, Qing Rao, Guo-Guang Zheng, Ke-Fu Wu, Expression and production of bioactive human interleukin-18 in transgenic tobacco plants Biotechnology Letters. ,vol. 25, pp. 1629- 1635 ,(2003) , 10.1023/A:1025638623770
Tomohiro Yoshimoto, Hitoshi Mizutani, Hiroko Tsutsui, Nancy Noben-Trauth, Kei-ichi Yamanaka, Minoru Tanaka, Shinzo Izumi, Haruki Okamura, William E. Paul, Kenji Nakanishi, IL-18 induction of IgE: dependence on CD4+ T cells, IL-4 and STAT6. Nature Immunology. ,vol. 1, pp. 132- 137 ,(2000) , 10.1038/77811
M Kurimoto, T Tanimoto, M Ikeda, K Konishi, K Hattori, Y Nukada, K Torigoe, T Okura, S Fukuda, M Fujii, K Akita, S Ushio, F Tanabe, M Namba, M Micallef, H Okamura, Cloning of the cDNA for human IFN-gamma-inducing factor, expression in Escherichia coli, and studies on the biologic activities of the protein. Journal of Immunology. ,vol. 156, pp. 4274- 4279 ,(1996)
Robert H. Wiltrout, Howard A. Young, Tomoaki Hoshino, IL-18 is a potent coinducer of IL-13 in NK and T cells: a new potential role for IL-18 in modulating the immune response. Journal of Immunology. ,vol. 162, pp. 5070- 5077 ,(1999)
E. Van Haute, H. Joos, M. Maes, G. Warren, M. Van Montagu, J. Schell, Intergeneric transfer and exchange recombination of restriction fragments cloned in pBR322: a novel strategy for the reversed genetics of the Ti plasmids of Agrobacterium tumefaciens. The EMBO Journal. ,vol. 2, pp. 411- 417 ,(1983) , 10.1002/J.1460-2075.1983.TB01438.X
Tae-Ho Kwon, Young-Mi Shin, Young-Sook Kim, Yong-Suk Jang, Moon-Sik Yang, Secretory production of hGM-CSF with a high specific biological activity by transgenic plant cell suspension culture Biotechnology and Bioprocess Engineering. ,vol. 8, pp. 135- 141 ,(2003) , 10.1007/BF02940269
Jae-Hwa Lee, Nguyen-Hoang Loc, Tae-Ho Kwon, Moon-Sik Yang, Partitioning of recombinant human granulocyte-macrophage colony stimulating factor (hGM-CSF) from plant cell suspension culture in PEG/sodium phosphate aqueous two-phase systems Biotechnology and Bioprocess Engineering. ,vol. 9, pp. 12- 16 ,(2004) , 10.1007/BF02949316
Feng Liu, James M. Lee, Effect of culture conditions on monoclonal antibody production from genetically modified tobacco suspension cultures Biotechnology and Bioprocess Engineering. ,vol. 4, pp. 259- 263 ,(1999) , 10.1007/BF02933749
Kaori Konishi, Fujimi Tanabe, Mutsuko Taniguchi, Hiroshi Yamauchi, Tadao Tanimoto, Masao Ikeda, Kunzo Orita, Masashi Kurimoto, A simple and sensitive bioassay for the detection of human interleukin-18/interferon-γ-inducing factor using human myelomonocytic KG-1 cells Journal of Immunological Methods. ,vol. 209, pp. 187- 191 ,(1997) , 10.1016/S0022-1759(97)00164-6
Janet M. Sharp, Pauline M. Doran, Effect of bacitracin on growth and monoclonal antibody production by tobacco hairy roots and cell suspensions Biotechnology and Bioprocess Engineering. ,vol. 4, pp. 253- 258 ,(1999) , 10.1007/BF02933748