Regulation of the epidermal growth factor receptor phosphorylation state by sphingosine in A431 human epidermoid carcinoma cells.
作者： M Faucher , N Gironès , Y A Hannun , R M Bell , R J Davis
DOI: 10.1016/S0021-9258(18)60718-5
关键词:
摘要: The regulation of protein phosphorylation by sphingosine in A431 human epidermoid carcinoma cells was examined. Sphingosine is a competitive inhibitor phorbol ester binding to kinase C (Ca2+/phospholipid-dependent enzyme) and potently inhibits phosphotransferase activity vitro. Addition intact caused an inhibition the ester-stimulated two substrates, epidermal growth factor (EGF) receptor threonine 654 transferrin serine 24. We conclude that cells. However, further experiments demonstrated sphingosine-treatment resulted EGF mechanism independent C. First, increase on unique tryptic peptide. Second, affinity Chinese hamster ovary expressing wild-type (Thr654) mutated (Ala654) receptors. also observed cause number EGF-binding sites expressed at surface Examination time course action effects were rapid (maximal 2 mins) prior stimulation 20 mins). bioactive molecule modulates cellular functions by: 1) inhibiting C; 2) stimulating C-independent pathway phosphorylation; 3) increasing cell
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