The AMeX method: A multipurpose tissue‐processing and paraffin‐embedding method. III. Extraction and purification of RNA and application to slot‐blot hybridization analysis
作者： Yuichi Sato , Kiyoshi Mukai , Shuichiroh Furuya , Yukio Shimosato
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摘要: RNA was extracted from tissues processed by a new fixation and paraffin-embedding method (the AMeX method) examined Northern blot analysis slot-blot analysis. The extraction for AMeX-processed tissue sections after the deparaffinization step same as that fresh materials. total amount of cellular mouse liver slightly less than tissue. In malignant lymphoma, 25 each 20 microns thick about 1.6-1.8 micrograms/mm2, regardless histological subtype period storage. moderately degraded, usually could not be used hybridization intensity ethidium bromide staining signals were reduced in comparison with material, but specific detected We have demonstrated previously preserves high-molecular-weight DNA various antigens. Since present study showed information on mRNA can obtained tissue, versatility usefulness this further proven.
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