Control of tick infestations in cattle vaccinated with bacterial membranes containing surface-exposed tick protective antigens

作者: Consuelo Almazán , Orlando Moreno-Cantú , Juan A. Moreno-Cid , Ruth C. Galindo , Mario Canales

DOI: 10.1016/J.VACCINE.2011.10.102

关键词:

摘要: Vaccines containing the Rhipicephalus (Boophilus) microplus BM86 and BM95 antigens protect cattle against tick infestations. Tick subolesin (SUB), elongation factor 1a (EF1a) ubiquitin (UBQ) are new candidate protective for control of Previous studies showed that R. immunogenic peptides fused to Anaplasma marginale major surface protein (MSP) N-terminal region (BM95-MSP1a) presentation on Escherichia coli membrane were infestations in rabbits. In this study, we extended these results by expressing SUB-MSP1a, EF1a-MSP1a UBQ-MSP1a fusion proteins E. using system demonstrating bacterial membranes chimeric BM95-MSP1a SUB-MSP1a (>60% vaccine efficacy) experimental annulatus cattle. This provides a novel, simple cost-effective approach production display antigenic chimera demonstrates possibility recombinant fractions preparations

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