Regional assignment of Y-linked DNA probes by deletion mapping and their homology with X- chromosome and autosomal sequences

摘要: A series of Y recombinants have been isolated from Y-specific DNA libraries and regionally located on the chromosome using a deletion panel constructed individuals carrying structural abnormalities chromosome. Of twenty examined twelve assigned to Yp eight Yq. Five map between Yp11.2 Ypter one can only be Yp. former, four detect homologies X Xq13 Xq24 latter Xp22.3 Xpter. The sixth recombinant detects autosomal homologous sequences. six remaining probes are Ycen Yp11.2. One these homology at Xq13-Xq24 sequences, two uniquely sequences three complex pattern homologies. intervals Yq11.21 additional Two lie in interval Yq11.21-Yq11-22, which other an Xp Finally, Yq all Yq11.22 Yq12. divergence different chromosomes has for by washing Southern Blots levels stringency. Additionally, analysis flow sorted used identify autosomes recombinants.