High-efficiency liquid chromatographic separation of vitamin D from precalciferol
作者： G.J. Krol , C.A. Mannan , F.Q. Gemmill , G.E. Hicks , B.T. Kho
DOI: 10.1016/S0021-9673(01)94971-4
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摘要: Abstract Vitamind D was separated from percalciferol within 1.2 min with a high-efficiency adsorptive liquid chromatographic suppor (Vydac) and low viscosity solvent. The efficiency of the system studied as function solvent flow-rate. In agreement Van Deemter's Giddings' equations for theoretical plate height, decreasing flow-rate enhanced column. However, in presence solvents, observed relationship between height could be interpreted best by equation eddy current mass transfer “coupling”. applied to (1) separation vitamin A acetate, palmitate, aldehyde, alcohol E (2) study cholecalciferol isomerization. isomerization equilibrium constants are greement previously published results.
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