A novel 40-kDa membrane-associated EF-hand calcium-binding protein in Plasmodium falciparum.
作者： D.J. Rawlings , D.C. Kaslow
DOI: 10.1016/S0021-9258(19)50621-4
关键词:
摘要: Abstract A 40-kDa sexual stage radiolabeled surface protein of Plasmodium falciparum, Pfs40, was previously identified as a potential target antigen transmission blocking immunity by an immunogenetic approach. Synthetic oligonucleotide "guessmers," based on microsequenced tryptic peptides Pfs40 purified two-dimensional gel electrophoresis, were used to clone the full length cDNA and genomic DNA encoding Pfs40. The deduced amino acid sequence predicted integral membrane containing five EF-hand calcium-binding domains. biological activity one or more these domains confirmed binding 45Ca both native recombinant Antisera immunoprecipitated protein. noncytosolic membrane-associated localization is unique within superfamily.
sci-hub.st 参考文章(41)
I A Quakyi, R Carter, L H Miller, N Kumar, J Rener, M F Good, The 230-kDa gamete surface protein of Plasmodium falciparum is also a target for transmission-blocking antibodies. Journal of Immunology. ,vol. 139, pp. 4213- 4217 ,(1987)
M Moos, N Y Nguyen, T Y Liu, Reproducible high yield sequencing of proteins electrophoretically separated and transferred to an inert support. Journal of Biological Chemistry. ,vol. 263, pp. 6005- 6008 ,(1988) , 10.1016/S0021-9258(18)68738-1
D C Kaslow, S Hill, Cloning metabolic pathway genes by complementation in Escherichia coli. Isolation and expression of Plasmodium falciparum glucose phosphate isomerase. Journal of Biological Chemistry. ,vol. 265, pp. 12337- 12341 ,(1990) , 10.1016/S0021-9258(19)38351-6
Richard Carter, Nirbhay Kumar, Isabella Quakyi, Michael Good, Kamini Mendis, Patricia Graves, Louis Miller, Immunity to Sexual Stages of Malaria Parasites Progress in allergy. ,vol. 41, pp. 193- 214 ,(1988) , 10.1159/000415224
K Tanabe, Ion metabolism in malaria-infected erythrocytes. Blood cells. ,vol. 16, pp. 437- 449 ,(1990)
P. Matsudaira, Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. Journal of Biological Chemistry. ,vol. 262, pp. 10035- 10038 ,(1987) , 10.1016/S0021-9258(18)61070-1
D.L. Urdal, D. Mochizuki, P.J. Conlon, C.J. March, M.L. Remerowski, J. Eisenman, C. Ramthun, S. Gillis, Lymphokine purification by reversed-phase high-performance liquid chromatography Journal of Chromatography A. ,vol. 296, pp. 171- 179 ,(1984) , 10.1016/S0021-9673(01)96410-6
T. Ifediba, J. P. Vanderberg, Complete in vitro maturation of Plasmodium falciparum gametocytes Nature. ,vol. 294, pp. 364- 366 ,(1981) , 10.1038/294364A0
G. Rigaud, T. Grange, R. Pictet, The use of NaOH as transfer solution of DNA onto nylon membrane decreases the hybridization efficiency. Nucleic Acids Research. ,vol. 15, pp. 857- 857 ,(1987) , 10.1093/NAR/15.2.857
Jack Kyte, Russell F. Doolittle, A simple method for displaying the hydropathic character of a protein Journal of Molecular Biology. ,vol. 157, pp. 105- 132 ,(1982) , 10.1016/0022-2836(82)90515-0