Antimicrobial proficiency testing of National Nosocomial Infections Surveillance System hospital laboratories.

摘要: Objective:The National Nosocomial Infections Surveillance (NNIS) System personnel report trends in antimicrobial-resistant pathogens. To validate select antimicrobial susceptibility testing results and to identify test methods that tend produce errors, we conducted proficiency among NNIS hospital laboratories.Setting:NNIS laboratories the United States.Methods:Each laboratory received five organisms (ie, an imipenem-resistant Serratia marcescens, oxacillin-resistant Staphylococcus aureus, a vancomycin-resistant Enterococcus faecalis, vancomycin-intermediate epidermidis, extended-spectrum beta-lactamase (ESβL)-producing Klebsiella pneumoniae). Testing were compared with reference from Centers for Disease Control Prevention.Results:Of 138 imipenem against marcescens strain, 110 (80%) correctly reported minimum inhibitory concentrations (MICs) or zone sizes resistant range. All 193 participating aureus strain as oxacillin resistant. Of laboratories, 169 (88%) correct MICs faecalis. One hundred sixty-two (84%) of demonstrated ability detect epidermidis; however, disk diffusion performed poorly when both staphylococci enterococci vancomycin. Although nonsusceptible cephalosporins aztreonam K. pneumoniae, only 98 (51%) this organism ESβL producer.Conclusion:Overall, detected most emerging resistance patterns. Disk continues be unreliable vancomycin must used cautiously enterococci. Further education on processing ESβL-producing is warranted.