Removal of micro-particles by microbial granules used for aerobic wastewater treatment.

摘要: Microbial granules with a diameter from 0.4 mm to 3.0 have been produced by fast sedimentation and retention of microbial aggregates in sequencing batch airlift reactors used for model wastewater treatment. The was or without addition calcium salt. were able not only degrade organic matter but remove nano- micro-particles due microchannels pores the matrix granules. To detect removal 0.1 μm, 0.6 4.2 μm fluorescent microspheres, cells Escherichia coli , stained permeable nucleic acid stain SYTO9™, incubated these particles. rate particle their accumulation measured Fluoview300 confocal laser scanning microscope (CLSM) (Olympus, Japan); FACSCalibur flow cytometer (Becton Dickinson, CA, USA), fluorescence spectrometer LS-50B (Perkin-Elmer, UK). release biological non-biological particles analyzed after DNA staining. Total number bigger than approximately 4 × 107 per ml, 23% bacterial cells. 4.2. microbeads accumulated within 250 upper layer granule externally added penetrated depth 800 addition. contained also attached ciliates protozoan smaller matrix. Kinetics sorption revealed cytometry spectrometry. Almost half E. can be removed one hour. ability enhance function aerobic treatment wastewater.