Enzymatic synthesis of novel oligosaccharides from L-sorbose, maltose, and sucrose using kojibiose phosphorylase.
作者： Hiroto Chaen , Tomoyuki Nishimoto , Tetsuya Nakada , Shigeharu Fukuda , Masashi Kurimoto
DOI: 10.1016/S1389-1723(01)80220-6
关键词:
摘要: Glucosyl-L-sorbose, -maltose, and -sucrose were synthesized using kojibiose phosphorylase (KPase) from Thermoanaerobacter brockii ATCC35047 with beta-D-glucose-1-phosphate (beta-G1P) as a glucosyl donor. One disaccharide two trisaccharides thus isolated by Toyopearl HW-40S column chromatography. The results of KPase digestion, methylation analysis, 13C-NMR studies indicated that these oligosaccharides alpha-D-glucopyranosyl-(1-->5)-alpha-L-sorbopyranose, alpha-D-glucopyranosyl-(1-->2)-alpha-D-glucopyranosyl-(1-->4)-D-glucopyranose (4-alpha-D-kojibiosyl-glucose), alpha-D-glucopyranosyl-(1-->2)-alpha-D-glucopyranosyl-(1-->2)-beta-D-fructofuranoside, which are all novel oligosaccharides. Glucosyl-L-sorbose was partially hydrolyzed to glucose L-sorbose alpha-glucosidases, while glucosyl-sucrose glucosyl-maltose not glucoamylase, or CGTase.
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