Cloning and increased expression of an insulin receptor substrate - 1-like gene in human hepatocellular carcinoma
作者： Masaki Nishiyama , Jack R. Wands
DOI: 10.1016/0006-291X(92)91640-C
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摘要: Abstract Human insulin receptor substrate-1 (hlRS-1) cDNAs were cloned from a λGT11 expression library using monoclonal antibody (MAb) produced against human hepatocellular carcinoma (HCC) cell line (FOCUS). The predicted amino acid sequence derived both genomic DNA fragment and the showed 90.5% identity to previously reported rat IRS-1 cDNA [Sun, X.P. (1991) Nature 352, 73–77]. Multiple potential phosphorylation sites, that suggest an intrinsic function of this molecule in response action, highly conserved between two species. A c.a. 180 kDa hlRS-1 protein was immunoprecipitated found be phosphorylated on tyrosine residue(s) following stimulation HuH-7 HCC cells. Northern blot analysis demonstrated single 5 kb transcript lines tissues. Higher levels gene transcripts observed tumors compared adjacent non-involved normal liver.
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